ecoli.processes.enzyme_kinetics
Convenience Kinetics
- class ecoli.processes.enzyme_kinetics.EnzymeKinetics(parameters=None)[source]
Bases:
Process
Michaelis-Menten-style enzyme kinetics model
- Parameters:
initial_parameters –
Configures the process with the following configuration options:
reactions (
dict
): Specifies the stoichiometry, reversibility, and catalysts of each reaction to model. For a non-reversible reaction \(A + B \rightleftarrows 2C\) catalized by an enzyme \(E\), we have the following reaction specification:{ # reaction1 is a reaction ID 'reaction1': { 'stoichiometry': { # 1 mol A is consumd per mol reaction ('internal', 'A'): -1, ('internal', 'B'): -1, # 2 mol C are produced per mol reaction ('internal', 'C'): 2, }, 'is reversible': False, 'catalyzed by': [ ('internal', 'E'), ], } }
Note that for simplicity, we assumed all the molecules and enzymes were in the
internal
port, but this is not necessary.kinetic_parameters (
dict
): Specifies the kinetics of the reaction by providing \(k_{cat}\) and \(K_M\) parameters for each enzyme. For example, let’s say that for the reaction described above, \(k{cat} = 1\), \(K_A = 2\), and \(K_B = 3\). Then the reaction kinetics would be specified by:{ 'reaction1': { ('internal', 'E'): { 'kcat_f': 1, # kcat for forward reaction ('internal', 'A'): 2, ('internal', 'B'): 3, }, }, }
If the reaction were reversible, we could have specified
kcat_r
as the \(k_{cat}\) of the reverse reaction.
- name = 'enzyme_kinetics'